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pei max transfection reagent  (Kyfora Bio)


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    Structured Review

    Kyfora Bio pei max transfection reagent
    Pei Max Transfection Reagent, supplied by Kyfora Bio, used in various techniques. Bioz Stars score: 99/100, based on 5795 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pei+max/PEI+MAX+Transfection+Reagent/custom%4024765-1%4010%2E1016%2Fj%2Eomtn%2E2026%2E103074
    Average 99 stars, based on 5795 article reviews
    pei max transfection reagent - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Suspension:

    Article Title: Cryo-EM structures of CENP-A nucleosomes isolated from human cells
    Article Snippet: .. The Expi293F cells (Thermo Fisher Scientific) were grown in suspension culture at a density of approximately 3 × 10 6 cells/ml using HE400AZ medium (GMEP), and Flag-tagged CENP-A was overexpressed with a pCMV-based expression plasmid using PEI MAX (Polysciences) and Opti-MEM reduced-serum medium (Thermo Fisher Scientific). ..

    Expressing:

    Article Title: Cryo-EM structures of CENP-A nucleosomes isolated from human cells
    Article Snippet: .. The Expi293F cells (Thermo Fisher Scientific) were grown in suspension culture at a density of approximately 3 × 10 6 cells/ml using HE400AZ medium (GMEP), and Flag-tagged CENP-A was overexpressed with a pCMV-based expression plasmid using PEI MAX (Polysciences) and Opti-MEM reduced-serum medium (Thermo Fisher Scientific). ..

    Article Title: TRIM21-mediated OGT degradation inhibits breast cancer tumorigenesis and metastasis
    Article Snippet: .. Lentiviral particles were produced by co-transfecting HEK293T cells with the expression plasmids and packaging plasmids psPAX2 (Addgene) and pMD2.G (Addgene) using PEI Max (Polysciences). .. The lentiviral vector used for knockdown (pLKO.1-puro) or overexpression (pCDH-CMVMCS-EF1-Puro) was purchased from Miaoling Biotech, China.

    Article Title: Identification of small-molecule inhibitors of the immune system repressor Regnase-1
    Article Snippet: Briefly, ~40,000 HEK293T cells were plated in 100 μL culture media of each well of a 96-well plate (Greiner Bio-One). .. The following day, cells were transfected with 25 ng expression (e.g. pCMV Regnase-1), 25 ng reporter (e.g. pGL3 Il6 3'UTR 1-400), and 2.5 ng normalization (pRL SV40) plasmids using 0.3 μL 1 mg/mL PEI MAX (Polysciences) in 25 μL Opti-MEM (Gibco) per well. .. The day after transfection, cells were inhibited by the indicated concentration of suramin or vehicle control using 2 μL 1 mg/mL PEI MAX in 10 μL Opti-MEM per well for 24 h and measured using the Dual-Luciferase Reporter System (Promega) according to the manufacturer's instructions.

    Plasmid Preparation:

    Article Title: Cryo-EM structures of CENP-A nucleosomes isolated from human cells
    Article Snippet: .. The Expi293F cells (Thermo Fisher Scientific) were grown in suspension culture at a density of approximately 3 × 10 6 cells/ml using HE400AZ medium (GMEP), and Flag-tagged CENP-A was overexpressed with a pCMV-based expression plasmid using PEI MAX (Polysciences) and Opti-MEM reduced-serum medium (Thermo Fisher Scientific). ..

    Article Title: Extracellular matrix–targeted TNC-scFv armoring enhances CAR T cell persistence and antitumor activity in solid tumors
    Article Snippet: .. Cotransfection was performed using PEI-MAX (24765, Polysciences, China) with CAR-expressing plasmids, the packaging plasmid psPAX2 (12260, Addgene, USA), and the envelope plasmid pMD2.G (12259, Addgene, USA) at an optimized ratio. ..

    Article Title: CHO cell production of a single, enveloped VLP vaccine co-displaying SARS-CoV-2, influenza and RSV antigens
    Article Snippet: .. Briefly, CHO cells were transfected at 2 × 106 cells/mL with 1 μg plasmid/mL and 5 μg PEI-MAX (Polysciences)/mL. ..

    Transfection:

    Article Title: Arginine methylation-dependent stabilization of SUV39H1 promotes breast cancer growth.
    Article Snippet: Suppressors of variegation 3-9 homolog 1 (SUV39H1), the enzyme responsible for establishing histone H3 lysine 9 trimethylation (H3K9me3) marks in heterochromatin, is frequently dysregulated in cancers.. However, the mechanisms underlying SUV39H1 dysregulation in breast cancer remain largely unclear.. Here, we report that protein arginine methyltransferase 1 (PRMT1) directly interacts with SUV39H1 and dimethylates it at arginine 378 (R378).

    Article Title: USP7 protects histone H1.2 from proteasome-mediated degradation to facilitate DNA repair and pancreatic neuroendocrine neoplasms progression.
    Article Snippet: Ubiquitin-specific protease 7 (USP7), a deubiquitinase, is involved in tumor progression.. However, its roles in pancreatic neuroendocrine neoplasms (pNENs) remain unclear.. The main objective of this study was therefore to investigate the molecular mechanism of how USP7 promoted pNEN progression.

    Article Title: Identification of small-molecule inhibitors of the immune system repressor Regnase-1
    Article Snippet: Briefly, ~40,000 HEK293T cells were plated in 100 μL culture media of each well of a 96-well plate (Greiner Bio-One). .. The following day, cells were transfected with 25 ng expression (e.g. pCMV Regnase-1), 25 ng reporter (e.g. pGL3 Il6 3'UTR 1-400), and 2.5 ng normalization (pRL SV40) plasmids using 0.3 μL 1 mg/mL PEI MAX (Polysciences) in 25 μL Opti-MEM (Gibco) per well. .. The day after transfection, cells were inhibited by the indicated concentration of suramin or vehicle control using 2 μL 1 mg/mL PEI MAX in 10 μL Opti-MEM per well for 24 h and measured using the Dual-Luciferase Reporter System (Promega) according to the manufacturer's instructions.

    Article Title: CHO cell production of a single, enveloped VLP vaccine co-displaying SARS-CoV-2, influenza and RSV antigens
    Article Snippet: .. Briefly, CHO cells were transfected at 2 × 106 cells/mL with 1 μg plasmid/mL and 5 μg PEI-MAX (Polysciences)/mL. ..

    Knockdown:

    Article Title: DHA elongates the neuronal primary cilium through GPR40-mediated signaling
    Article Snippet: .. Knockdown efficiency was validated in HEK293T cells co-transfected with GPR40-EGFP and either the control or one of the two Ffar1 RNAi vectors using PEI MAX (Polysciences). .. Cells were lysed in 1× RIPA buffer 48 h after transfection, protein concentrations were determined using a BCA protein assay kit (Fujifilm Wako Pure Chemical), and 15 μg of protein per lane was resolved by SDS-PAGE.

    Control:

    Article Title: DHA elongates the neuronal primary cilium through GPR40-mediated signaling
    Article Snippet: .. Knockdown efficiency was validated in HEK293T cells co-transfected with GPR40-EGFP and either the control or one of the two Ffar1 RNAi vectors using PEI MAX (Polysciences). .. Cells were lysed in 1× RIPA buffer 48 h after transfection, protein concentrations were determined using a BCA protein assay kit (Fujifilm Wako Pure Chemical), and 15 μg of protein per lane was resolved by SDS-PAGE.

    Cotransfection:

    Article Title: Extracellular matrix–targeted TNC-scFv armoring enhances CAR T cell persistence and antitumor activity in solid tumors
    Article Snippet: .. Cotransfection was performed using PEI-MAX (24765, Polysciences, China) with CAR-expressing plasmids, the packaging plasmid psPAX2 (12260, Addgene, USA), and the envelope plasmid pMD2.G (12259, Addgene, USA) at an optimized ratio. ..

    Produced:

    Article Title: TRIM21-mediated OGT degradation inhibits breast cancer tumorigenesis and metastasis
    Article Snippet: .. Lentiviral particles were produced by co-transfecting HEK293T cells with the expression plasmids and packaging plasmids psPAX2 (Addgene) and pMD2.G (Addgene) using PEI Max (Polysciences). .. The lentiviral vector used for knockdown (pLKO.1-puro) or overexpression (pCDH-CMVMCS-EF1-Puro) was purchased from Miaoling Biotech, China.



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    Image Search Results


    Journal: iScience

    Article Title: An inverse-protein-folding pre-trained model for predicting the mutational effects in protein-protein interactions

    doi: 10.1016/j.isci.2026.117693

    Figure Lengend Snippet:

    Article Snippet: polyethyleneimine (PEI) , Polysciences , Cat# 24765-1.

    Techniques: Virus, Recombinant, Software, Analysis, Enzyme-linked Immunosorbent Assay